Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22065-1-AP targets LTBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17141 Product name: Recombinant human LTBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 455-804 aa of BC130289 Sequence: VEALTFSREHGPGVAEPEVATAPPEKEIPSLDQEKTKLEPGQPQLSPGISTIHLHPQFPVVIEKTSPPVPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYRFSEQQRKCVDIDECTQVQHLCSQGRCENTEGSFLCICPAGFMASEEGTNCIDVDECLRPDVCGEGHCVNTVGAFRCEYCDSGYRMTQRGRCEDIDECLNPSTCPDEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLEPNVCANGDCSNLEGSYMCSCHKGYTRTPDHKHCRDIDECQQGNLCVNGQCKNTEGSFRCTCGQGYQLSAAKDQCEDIDECQHRHLC Predict reactive species |
| Full Name | latent transforming growth factor beta binding protein 1 |
| Calculated Molecular Weight | 1721 aa, 187 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC130289 |
| Gene Symbol | LTBP1 |
| Gene ID (NCBI) | 4052 |
| RRID | AB_11232219 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) is a large extracellular matrix protein that belongs to the latent TGF-beta binding protein family. It plays a crucial role in the regulation of TGF-beta, a multifunctional cytokine involved in various cellular processes such as cell growth, differentiation, and immune response. LTBP1 is essential for TGF-beta folding, secretion, matrix localization, and activation. It forms latent complexes with TGF-beta by covalently binding the TGF-beta propeptide (LAP) via disulfide bonds in the endoplasmic reticulum. This complex is then secreted and targeted to the extracellular matrix, where TGF-beta can be activated by various mechanism
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LTBP1 antibody 22065-1-AP | Download protocol |
| WB protocol for LTBP1 antibody 22065-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LTBP1 Polyclonal antibody (Cat# 22065-1-AP) has 2 citations. They appear in Cardiovascular Pathology (2025) and Matrix Biology Plus (2026). The research fields encompass cardiovascular pathology—specifically arrhythmogenic cardiomyopathy and calcific aortic valve disease—and extracellular matrix biology, focusing on matrix remodeling and collagen organization. Cited applications include IF and IHC in human samples.
| Species | Application | Title |
|---|---|---|
Cardiovasc Pathol Molecular Features and Cell Composition of Left-Dominant Arrhythmogenic Cardiomyopathy Reveals Key Pathways and Therapeutic Targets | ||
Matrix Biol Plus Extracellular matrix remodeling in calcific aortic valve disease: Localized enrichment of type III collagen and LTBP-4. |
Reviews
What customers say
All three reviews rate this LTBP1 Rabbit Polyclonal Antibody 5 stars for Western Blot. Users report consistent, validated results with no issues encountered over time. One reviewer used a 1:1000 dilution on a cancer cell line and confirmed reliable performance. Others tested it on melanoma cell lines, noting good blotting results and dependable quality across multiple uses. Overall, customers find the antibody effective, reproducible, and trouble-free for detecting LTBP1 in Western Blot applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Haley (Verified Customer) (12-01-2025) | Validated results
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jelan (Verified Customer) (10-16-2025) | LTBP1 Rabbit PolyAb, we've been using this antibody and have not faced any issues for it in the past, and weve gotten good blotting results.
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jelan (Verified Customer) (10-16-2025) | LTBP1 Rabbit PolyAb, we've been using this antibody and have not faced any issues for it in the past, and weve gotten good blotting results.
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