LTF Polyclonal antibody

• Featured Product
• KD/KO Validated

LTF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10933-1-AP

Synonyms

GIG12, HLF2, Lactoferrin, lactotransferrin, LF, LTF, Talalactoferrin

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Validation Data Gallery

• 

  WB analysis using 10933-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10933-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 10933-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10933-1-AP (Lactoferrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10933-1-AP

  IP result of anti-LTF (IP:10933-1-AP, 4ug; Detection:10933-1-AP 1:600) with HeLa cells lysate 4000ug.

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  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10933-1-AP (Lactoferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 10933-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10933-1-AP (Lactoferrin antibody at dilution of 1:50(under 40x lens).

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Tested Applications

• Positive WB detected in: human saliva, HEK-293 cells, human pancreas tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Product Information

10933-1-AP targets LTF in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTF fusion protein Ag1379
• Full Name: lactotransferrin
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 30,51,80 kDa
• GenBank Accession Number: BC015822
• Gene Symbol: LTF
• Gene ID (NCBI): 4057
• RRID: AB_2139227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).

Publications

Species	Application	Title
human	IHC	Sci Adv; Mucus concentration-dependent biophysical abnormalities unify submucosal gland and superficial airway dysfunction in cystic fibrosis.; Authors - Takafumi Kato
human,mouse	WB	Clin Exp Immunol; C/EBPε and its acetylation in PMN enhance the tolerance to trauma; Authors - Shaowen Cheng
human	WB,IHC	Cancer Cell Int; Identification and validation of an immune-related prognostic signature and key gene in papillary thyroid carcinoma.; Authors - Rujia Qin
human	WB	Biochem Biophys Res Commun; NEDD4L-mediated LTF protein degradation limits ferroptosis.; Authors - Yuan Wang ; KD Validated
human	IHC	Int Med Case Rep J; Collagen fiber changes related to keratoconus with secondary corneal amyloidosis.; Authors - Kaoru Araki-Sasaki
mouse	WB	Bioact Mater; Passively-targeted mitochondrial tungsten-based nanodots for efficient acute kidney injury treatment; Authors - Qiong Huang