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LUC7L Polyclonal antibody

LUC7L Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17085-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

LUC7 LIKE, LUC7 like (S. cerevisiae), LUC7B1, LUC7L, LUC7L1, Putative SR protein LUC7B1, SR+89

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse brain using 17085-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 17085-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 17085-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse liver using 17085-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 17085-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17085-1-AP (LUC7L antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 17085-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17085-1-AP (LUC7L antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse liver tissue
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17085-1-AP targets LUC7L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9170 Product name: Recombinant human LUC7L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-354 aa of BC065198 Sequence: MQMGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLESSNGKMASRRSEEKEAGEI Predict reactive species
Full Name	LUC7-like (S. cerevisiae)
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	44 kDa
GenBank Accession Number	BC065198
Gene Symbol	LUC7L
Gene ID (NCBI)	55692
RRID	AB_2878346
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NQ29
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for LUC7L antibody 17085-1-AP	Download protocol
WB protocol for LUC7L antibody 17085-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LUC7L Polyclonal antibody (Cat# 17085-1-AP) has 2 total citations. One appears in Cell Reports (2021), studying functional analyses of human LUC7-like proteins in splicing regulation and myeloid neoplasms. The other is in Journal of Biological Chemistry (2021), investigating phosphorylation regulation of arginine-rich RNA-binding protein solubility and oligomerization. Both studies used the antibody for Western blot in human samples, covering research fields of RNA biology, splicing, and cancer.

Species	Application	Title
human	WB	Cell Rep Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms. Authors - Noah J Daniels KD Validated View Article
Human	WB	J Biol Chem Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization. Authors - Sean R Kundinger View Article