LUC7L Polyclonal antibody

• Featured Product
• KD/KO Validated

LUC7L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17085-1-AP

Synonyms

LUC7 LIKE, LUC7 like (S. cerevisiae), LUC7B1, LUC7L, LUC7L1, Putative SR protein LUC7B1, SR+89

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Validation Data Gallery

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  WB analysis of mouse brain using 17085-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17085-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17085-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17085-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17085-1-AP (LUC7L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 17085-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17085-1-AP (LUC7L antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 17085-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17085-1-AP (LUC7L antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 17085-1-AP is LUC7L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LUC7L fusion protein Ag9170
• Full Name: LUC7-like (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC065198
• Gene Symbol: LUC7L
• Gene ID (NCBI): 55692
• RRID: AB_2878346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.; Authors - Noah J Daniels ; KD Validated