LY86 Recombinant monoclonal antibody

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• LY86 Recombinant monoclonal antibody (87173-1-RR)

LY86 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 87173-1-RR Copied!

Clone No. 252168G12

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1

• 

  WB analysis using 87173-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87173-1-RR (LY86 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 87173-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87173-1-RR (LY86 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of THP-1 using 87173-1-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using LY86 antibody (87173-1-RR, Clone: 252168G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87173-1-RR targets LY86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3108

  Product name: recombinant human LY86 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-162 aa of NM_004271.4

  Sequence: GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS

• Full Name: lymphocyte antigen 86
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: NM_004271.4
• Gene Symbol: LY86
• Gene ID (NCBI): 9450
• RRID: AB_3745388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95711
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LY86, also known as protein MD-1, is a secreted glycoprotein that is associated with RP105 (TLR family proteins) and plays a significant role in the B cell surface expression of RP105. The RP105/MD-1 complex is expressed on immune cells, including B cells, macrophages, and dendritic cells (PMID: 37861500). By forming the RP105-MD-1 complex, LY86 fine-tunes recognition of bacterial lipopolysaccharide (LPS) and subsequent cytokine production, acting as both a facilitator and a brake on innate immune responses. LY86 influences sterile inflammatory conditions such as atherosclerosis, obesity, insulin resistance and cardiac remodeling, and is being explored as a biomarker for unstable carotid plaques and other immune-mediated diseases (PMID: 39085350).