Anti-Human LY86 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 98678-1-RR (same clone as 98678-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human LY86 Rabbit RecAb (98678-1-RR, Clone:252168G12), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Lymphocytes were gated. This data was developed using the same antibody clone with 98678-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98678-1-RR (same clone as 98678-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human LY86 Rabbit RecAb (98678-1-RR, Clone:252168G12)(red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). Lymphocytes were gated. This data was developed using the same antibody clone with 98678-1-PBS in a different storage buffer formulation.

LY86 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98678-1-PBS

Clone No.252168G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Ly 86, lymphocyte antigen 86, MD 1, MD1, MMD 1

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Product Information

98678-1-PBS targets LY86 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3108

  Product name: recombinant human LY86 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 21-162 aa of NM_004271.4

  Sequence: GGGGKAWPTHVVCSDSGLEVLYQSCDPLQDFGFSVEKCSKQLKSNINIRFGIILREDIKELFLDLALMSQGSSVLNFSYPICEAALPKFSFCGRRKGEQIYYAGPVNNPEFTIPQGEYQVLLELYTEKRSTVACANATIMCS

• Full Name: lymphocyte antigen 86
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_004271.4
• Gene Symbol: LY86
• Gene ID (NCBI): 9450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95711
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LY86, also known as protein MD-1, is a secreted glycoprotein that is associated with RP105 (TLR family proteins) and plays a significant role in the B cell surface expression of RP105. The RP105/MD-1 complex is expressed on immune cells, including B cells, macrophages, and dendritic cells (PMID: 37861500). By forming the RP105-MD-1 complex, LY86 fine-tunes recognition of bacterial lipopolysaccharide (LPS) and subsequent cytokine production, acting as both a facilitator and a brake on innate immune responses. LY86 influences sterile inflammatory conditions such as atherosclerosis, obesity, insulin resistance and cardiac remodeling, and is being explored as a biomarker for unstable carotid plaques and other immune-mediated diseases (PMID: 39085350).