Lysozyme Polyclonal antibody

Lysozyme Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (2)

Cat no : 15013-1-AP

Synonyms

LYZ, Muramidase, LZM, Lysozyme C, EC:3.2.1.17

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Validation Data Gallery

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  WB analysis of THP-1 using 15013-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (Lysozyme antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15013-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 15013-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15013-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse lung tissue, human spleen tissue, HL-60 cells
• Positive IHC detected in: mouse heart tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 5 publications below

Product Information

15013-1-AP targets Lysozyme in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lysozyme fusion protein Ag6917
• Full Name: lysozyme (renal amyloidosis)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 15-17 kDa
• GenBank Accession Number: BC004147
• Gene Symbol: Lysozyme
• Gene ID (NCBI): 4069
• RRID: AB_10639507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publications

Species	Application	Title
		Cell Chem Biol; Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.; Authors - Sara H Rouhanifard
human		Arch Toxicol; Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.; Authors - C Biel
mouse	WB	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
mouse	WB	Arch Med Sci; Lysozyme promotes renal fibrosis through the JAK/STAT3 signal pathway in diabetic nephropathy; Authors - Yan Ren
mouse		Int J Mol Sci; Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.; Authors - Wenyan Yang
rat	IF	Physiol Genomics; The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.; Authors - Kari Chesney

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cell line (caco2 cells)

FH

Claudio (Verified Customer) (12-15-2021)

The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse heart sections