LZTS2 Polyclonal antibody

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  WB analysis using 15677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15677-1-AP (LZTS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15677-1-AP

  WB result of LZTS2 antibody (15677-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LZTS2 transfected HeLa cells.

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  IHC staining of human prostate hyperplasia using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

LZTS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15677-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Synonyms

hLZTS2, KIAA1813, LAPSER1, LZTS2, Protein LAPSER1

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells
• Positive IHC detected in: human prostate hyperplasia tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

15677-1-AP targets LZTS2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8145

  Product name: Recombinant human LZTS2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 319-669 aa of BC006212

  Sequence: PSDEALLHCVLEGKLRDREAELQQLRDSLDENEATMCQAYEERQRHWQREREALREDCAAQAQRAQRAQQLLQLQVFQLQQEKRQLQDDFAQLLQEREQLERRCATLEREQRELGPRLEETKWEVCQKSGEISLLKQQLKESQAELVQKGSELVALRVALREARATLRVSEGRARGLQEAARARELELEACSQELQRHRQEAEQLREKAGQLDAEAAGLREPPVPPATADPFLLAESDEAKVQRAAAGVGGSLRAQVERLRVELQRERRRGEEQRDSFEGERLAWQAEKEQVIRYQKQLQHNYIQMYRRNRQLEQELQQLSLELEARELADLGLAEQAPCICLEEITATEI

• Full Name: leucine zipper, putative tumor suppressor 2
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC006212
• Gene Symbol: LZTS2
• Gene ID (NCBI): 84445
• RRID: AB_2878166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BRK4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LZTS2, also named as KIAA1813 and LAPSER1, is a novel b-Catenin-interacting protein and it regulates the nuclear export of b-Catenin. LZTS2 is a negative regulator of katanin-mediated microtubule severing and release from the centrosome. It is required for central spindle formation and the completion of cytokinesis.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Nat Commun; Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.; Authors - Yohendran Baskaran
human	WB,IP	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu ; KD Validated
human	WB	Cancer Lett; LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85.; Authors - Shuangbing Xu ; KD Validated
human	WB,IF	bioRxiv; α-catenin mechanosensitivity as a route to cytokinesis failure through sequestration of LZTS2; Authors - Yuou Wang ; KD Validated
human	WB,IP	Cell Signal; Phosphorylation of LZTS2 by PLK1 activates the Wnt pathway; Authors - Ran Liu
human	WB	Cell Death Dis; RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression; Authors - Zhixiong Dong