Tested Applications
| Positive WB detected in | HepG2 cells, RAW 264.7 cells, HeLa cells, HUVEC cells, A431 cells, SKOV-3 cells, HSC-T6 cells, NIH/3T3 cells, C6 cells |
| Positive IHC detected in | mouse heart tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81042-1-RR targets Lamin A/C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0408 Product name: Recombinant human Lamin A/C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-198 aa of BC003162 Sequence: ITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQ Predict reactive species |
| Full Name | lamin A/C |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa, 70 kDa |
| GenBank Accession Number | BC003162 |
| Gene Symbol | Lamin A/C |
| Gene ID (NCBI) | 4000 |
| ENSEMBL Gene ID | ENSG00000160789 |
| RRID | AB_2923698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02545 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| IHC protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| WB protocol for Lamin A/C antibody 81042-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Lamin A/C Recombinant monoclonal antibody (Cat# 81042-1-RR) has 7 citations published in Cell Death & Disease, Cancer Letters, International Journal of Biological Sciences, International Journal of Molecular Sciences, Frontiers in Pharmacology, and bioRxiv. Research fields span ovarian cancer, pancreatic cancer ferroptosis, coronary microembolization, progeria/HGPS, gastric intestinal metaplasia, and nuclear envelope dynamics during mitosis.
| Species | Application | Title |
|---|---|---|
Cell Death Dis MAP2K6 reprograms NAD(+) metabolism via the HMGA1-NAMPT axis to sensitize ovarian cancer cells to PARP inhibition. | ||
Cancer Lett SIK1 promotes ferroptosis resistance in pancreatic cancer via HDAC5-STAT6-SLC7A11 axis | ||
Int J Biol Sci De-succinylation-induced accumulation of TRMT10C in the nucleus plays a detrimental role in coronary microembolization via its m1A modification function | ||
Int J Mol Sci Inflammation and Fibrosis in Progeria: Organ-Specific Responses in an HGPS Mouse Model | ||
Int J Mol Sci Baricitinib and Lonafarnib Synergistically Target Progerin and Inflammation, Improving Lifespan and Health in Progeria Mice | ||
Front Pharmacol Rabeprazole suppressed gastric intestinal metaplasia through activation of GPX4-mediated ferroptosis |
Reviews
What customers say
Both reviewers rated this antibody 5 stars for Western Blot. One user achieved clear results at a 1:100,000 dilution in brown adipocytes with 5% milk blocking. The other obtained two distinct bands at the expected molecular weight (70–55 kDa) with no background noise at a 1:50,000 dilution in mesenchymal stem cells. Overall, users report strong specificity, clean signals, and reliable performance at high dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lukas (Verified Customer) (09-07-2026) | Western Blot 1:100000, Blocking with 5% Milk
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Charlotte (Verified Customer) (02-19-2026) | We have two clear bands at the expected size (70-55). No noise
 |
