Lamin B2 Monoclonal antibody, PBS Only

• 

  WB analysis using 68475-1-Ig (same clone as 68475-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68475-1-Ig (Lamin B2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 68475-1-Ig (same clone as 68475-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (68475-1-Ig, Clone: 2C7B1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68475-1-PBS in a different storage buffer formulation.

Lamin B2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68475-1-PBS

Clone No.2C7B1

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

LMNB2, LMN2, Lamin-B2, LAMB2, 2C7B1

---

Product Information

68475-1-PBS targets Lamin B2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33296

  Product name: Recombinant human LMNB2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 241-600 aa of BC006551

  Sequence: EELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM

• Full Name: lamin B2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC006551
• Gene Symbol: Lamin B2
• Gene ID (NCBI): 84823
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q03252
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).