Luciferase Polyclonal antibody

Luciferase Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

firefly, recombinant protein and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 27986-1-AP

Synonyms

Luciferin 4-monooxygenase

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Validation Data Gallery

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  WB analysis of Transfected HEK-293 using 27986-1-AP

  WB result of Luciferase antibody (27986-1-AP; 1:1000; room temperature for 1.5 hours) with negative control and Luciferase overexpressed Transfected HEK-293 cells.

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Tested Applications

• Positive WB detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

27986-1-AP targets Luciferase in WB, IHC, ELISA applications and shows reactivity with firefly, recombinant protein samples.

• Tested Reactivity: firefly, recombinant protein
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Luciferase fusion protein Ag27633
• Full Name: Luciferase
• RRID: AB_2881031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light. This antibody detects the Luciferase protein of Photinus pyralis (Common eastern firefly) (Lampyris pyralis).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh
human	WB	J Virol; SARS-CoV-2 NSP12 Protein Is Not an Interferon-β Antagonist.; Authors - Aixin Li
	WB	Nat Commun; A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity; Authors - Jiahui Liu
human	WB	J Virol; SARS-CoV-2 nsp13 Restricts Episomal DNA Transcription without Affecting Chromosomal DNA; Authors - Aixin Li
mouse	IHC	Acta Pharmacol Sin; Aiphanol, a multi-targeting stilbenolignan, potently suppresses mouse lymphangiogenesis and lymphatic metastasis; Authors - Shan-Mei Chen