Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27986-1-AP targets Luciferase in WB, IHC, ELISA applications and shows reactivity with firefly, recombinant protein samples.
| Tested Reactivity | firefly, recombinant protein |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27633 Product name: Recombinant photinus pyralis Luciferin 4 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: MTPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL Predict reactive species |
| Full Name | Luciferase |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881031 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08659 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Luciferase produces green light with the wavelength of 562 nm. Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light. This antibody detects the Luciferase protein of Photinus pyralis (Common eastern firefly) (Lampyris pyralis).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Luciferase antibody 27986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Luciferase Polyclonal antibody (Cat# 27986-1-AP) has 15 citations spanning journals including Nature Cell Biology, Nature Communications, EMBO Journal, Current Biology, Journal of Virology, Acta Pharmacologica Sinica, Communications Biology, STAR Protocols, Molecular Therapy Advances, NPJ Biology of Sleep, International Journal of Biological Macromolecules, and Journal of Integrative Plant Biology. Research fields cover cancer biology, virology, RNA/translation, circadian rhythms, plant immunity, and gene therapy.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics. | ||
Nat Commun A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity | ||
Nat Commun Simultaneous in vitro expression of minimal 21 transfer RNAs by tRNA array method. | ||
Acta Pharmacol Sin Aiphanol, a multi-targeting stilbenolignan, potently suppresses mouse lymphangiogenesis and lymphatic metastasis | ||
Int J Biol Macromol Rational design of a cost-effective IRES-stem-loop translation system independent of cap and poly(A) tail | ||
EMBO J A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression. |
