Anti-Mouse Ly-6C (HK1.4) Rat IgG2a Recombinant Antibody, PBS Only

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  FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1), and FITC anti-Rat IgG2a antibody. Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed. This data was developed using the same antibody clone with 65669-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse bone marrow cells using 65669-1-TR (same clone as 65669-1-PBS)

  1x10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6C (HK1.4) Rat IgG2a RecAb (65669-1-TR, Clone: HK1.4) (red) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed. This data was developed using the same antibody clone with 65669-1-PBS in a different storage buffer formulation.

Ly-6C Uni-rAb^TM Recombinant Antibody for FC

Cat No. 65669-1-PBS

Clone No.HK1.4

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

Ly6c, Ly 6C, Ly6c1

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Product Information

65669-1-PBS targets Ly-6C in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: lymphocyte antigen 6 complex, locus C1
• GenBank Accession Number: BC010764
• Gene Symbol: Ly-6C
• Gene ID (NCBI): 17067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).