Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65140-1-Ig targets Ly-6G/Ly-6C (Gr-1) in WB, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Raised against granulocytes of mouse origin Predict reactive species |
| Full Name | lymphocyte antigen 6 complex, locus G |
| GenBank Accession Number | X70920 |
| Gene Symbol | Ly-6G |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2881475 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35461 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Ly-6G/Ly-6C (Gr-1) antibody 65140-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5), catalog number 65140-1-Ig, has 35 citations across journals including Nature Communications, Science Advances, Cell Reports, Biomaterials, Journal of Leukocyte Biology, Life Sciences, International Journal of Biological Sciences, Acta Pharmacologica Sinica, and others. Research fields span neutrophil biology and NETosis, inflammatory diseases, tumor microenvironment, liver and lung injury, neuroinflammation, wound healing, pancreatitis, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Nat Commun Unimolecular near-infrared chemiluminescent reporter for cascaded multiplex imaging of ischemia-reperfusion injury in the liver-kidney axis | ||
Nat Commun Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats | ||
J Nanobiotechnology Citrate-coated Prussian blue nanozyme hitchhikes neutrophils to ameliorate calcium oxalate crystal-induced kidney injury via inhibiting pyroptosis and NETosis. | ||
MedComm (2020) BCR-ABL1 Drives Transcriptional Reprogramming of Chronic Myeloid Leukemia Cells for Immune Evasion Through C/EBPβ. | ||
Sci Adv Silicate-based therapy for inflammatory dilated cardiomyopathy by inhibiting the vicious cycle of immune inflammation via FOXO signaling | ||
Biomaterials Integrated-omics profiling unveils the disparities of host defense to ECM scaffolds during wound healing in aged individuals |
