Anti-Mouse Ly-6G (Gr-1) (RB6-8C5)

Ly-6G (Gr-1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

RB6-8C5

Cat no : 65140-1-Ig

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G (Gr-1)



Tested Applications

Positive FC detected inmouse bone marrow cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65140-1-Ig targets Ly-6G (Gr-1) in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Raised against granulocytes of mouse origin
Full Name lymphocyte antigen 6 complex, locus G
GenBank accession numberX70920
Gene symbol Ly6g
Gene ID (NCBI) 546644
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767).

Protocols

Product Specific Protocols
FC protocol for Ly-6G (Gr-1) antibody 65140-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Mol Cell Cardiol

The nuclear receptor co-repressor 1 is a novel cardioprotective factor against acute myocardial ischemia-reperfusion injury.

Authors - Zihan Qin
mouseIF,FC

J Leukoc Biol

The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis.

Authors - Yitong Liu
mouse

J Oncol

Blocking TGF-β Expression Attenuates Tumor Growth in Lung Cancers, Potentially Mediated by Skewing Development of Neutrophils.

Authors - Yifei Fang
mouseIF

Gut Microbes

Bifidobacterium spp. and their metabolite lactate protect against acute pancreatitis via inhibition of pancreatic and systemic inflammatory responses

Authors - Han Li