Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5)

• 

  FC experiment of mouse bone marrow cells using 65140-1-Ig

  1X10^6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD11b and 0.5 ug Anti-Mouse Ly-6G/Ly-6C (Gr-1) (65140-1-Ig, Clone:RB6-8C5) and FITC anti-Rat IgG2b Antibody at dilution 1:1000, or 0.5 ug Rat IgG2b isotype control. Cells were not fixed.

Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody for FC

Cat No. 65140-1-Ig

Clone No.RB6-8C5

Publications(23)

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

WB, FC

Synonyms

Gr-1, Ly6g, Ly 6G, Ly-6G, Ly-6G.1

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• FC: See 5 publications below

Product Information

65140-1-Ig targets Ly-6G/Ly-6C (Gr-1) in WB, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Raised against granulocytes of mouse origin

• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly-6G
• Gene ID (NCBI): 546644
• RRID: AB_2881475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P35461
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The RB6-8C5 mAb has been reported to cross-react with Ly-6C on cells expressing Ly-6C (PMID: 8360469).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse		CNS Neurosci Ther; Endothelial Changes at Different Stages After Ischemic Stroke Contribute to the Regulation of Immune Cell Infiltration; Authors - Xuejiao Dai
mouse	FC	J Leukoc Biol; The effect of an isoquinoline alkaloid on treatment of periodontitis by regulating the neutrophils chemotaxis.; Authors - Yitong Liu
mouse	FC	J Oncol; Blocking TGF-β Expression Attenuates Tumor Growth in Lung Cancers, Potentially Mediated by Skewing Development of Neutrophils.; Authors - Yifei Fang
mouse		Int Immunopharmacol; Aerosol inhalation of rhIL-10 improves acute lung injury in mice by affecting pulmonary neutrophil phenotypes through neutrophil-platelet aggregates; Authors - Huan Qin
mouse		J Transl Med; Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation; Authors - Zi-Yi Zhang
	FC	Oncol Rep; BCL2A1‑ and G0S2‑driven neutrophil extracellular traps: A protective mechanism linking preeclampsia to reduced breast cancer risk; Authors - Lu Xiao