MACC1 Polyclonal antibody

MACC1 Polyclonal Antibody for IHC, ELISA

Cat No. 21970-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

7A5, MACC1, SH3BP4L

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllIHC

IHC staining of human liver cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 21970-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21970-1-AP (MACC1 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in

human liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21970-1-AP targets MACC1 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16884 Product name: Recombinant human MACC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 502-852 aa of BC137090 Sequence: ITTPDPTPNLKRLSNLPGYLQKKEEIKSAPLSPKILVKYPTFQDKTLNFSNYGVTLKAVLRQSKIDYFLEYFKGDTIALLGEGKVKAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMFMSDSVFTTRNLLEQIVLPLKKLTYIYSVVLTLVSEKVYDWKVLADVLGYSHLSLEDFDQIQADKESEKVSYVIKKLKEDCHTERNTRKFLYELIVALLKMDCQELVARLIQEAAVLTSAVKLGKGWRELAEKLVRLTKQQMEAYEIPHRGNTGDVAVEMMWKPAYDFLYTWSAHYGNNYRDVLQDLQSALDRMKNPVTKHWRELTGVLILVNSLEVLRVTAFSTSEEV Predict reactive species
Full Name	metastasis associated in colon cancer 1
Calculated Molecular Weight	852 aa, 97 kDa
GenBank Accession Number	BC137090
Gene Symbol	MACC1
Gene ID (NCBI)	346389
RRID	AB_2878957
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZN28
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MACC1, Metalloantigenolytic Cleavage Cartilage Proteinase 1, belongs to the ADAM (a disintegrin and metalloprotease) family. MACC1 was first identified in colon cancer. It is a key prognostic biomarker and is involved in recurrence, metastasis, and survival in solid cancers (PMID: 23815185). MACC1 expression is primarily observed in cartilage, but it's also present in other tissues like bone, synovium, and the developing nervous system (PMID: 36979146).

Protocols

Product Specific Protocols
IHC protocol for MACC1 antibody 21970-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The MACC1 Polyclonal antibody (Cat# 21970-1-AP) has 2 total citations. These appear in International Journal of Medical Sciences and Molecular Carcinogenesis. The cited research covers esophageal squamous cell carcinoma prognostic modeling and endometrial carcinoma tumorigenesis and progression.

Species	Application	Title
human	IHC	Int J Med Sci Bioinformatics Analysis Reveals a Novel Prognostic Model for Esophageal Squamous Cell Carcinoma Authors - Huan Yang View Article
		Mol Carcinog The role of metastasis-associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression. Authors - Shuo Chen View Article