Tested Applications
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
21970-1-AP targets MACC1 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16884 Product name: Recombinant human MACC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 502-852 aa of BC137090 Sequence: ITTPDPTPNLKRLSNLPGYLQKKEEIKSAPLSPKILVKYPTFQDKTLNFSNYGVTLKAVLRQSKIDYFLEYFKGDTIALLGEGKVKAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMFMSDSVFTTRNLLEQIVLPLKKLTYIYSVVLTLVSEKVYDWKVLADVLGYSHLSLEDFDQIQADKESEKVSYVIKKLKEDCHTERNTRKFLYELIVALLKMDCQELVARLIQEAAVLTSAVKLGKGWRELAEKLVRLTKQQMEAYEIPHRGNTGDVAVEMMWKPAYDFLYTWSAHYGNNYRDVLQDLQSALDRMKNPVTKHWRELTGVLILVNSLEVLRVTAFSTSEEV Predict reactive species |
| Full Name | metastasis associated in colon cancer 1 |
| Calculated Molecular Weight | 852 aa, 97 kDa |
| GenBank Accession Number | BC137090 |
| Gene Symbol | MACC1 |
| Gene ID (NCBI) | 346389 |
| RRID | AB_2878957 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZN28 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MACC1, Metalloantigenolytic Cleavage Cartilage Proteinase 1, belongs to the ADAM (a disintegrin and metalloprotease) family. MACC1 was first identified in colon cancer. It is a key prognostic biomarker and is involved in recurrence, metastasis, and survival in solid cancers (PMID: 23815185). MACC1 expression is primarily observed in cartilage, but it's also present in other tissues like bone, synovium, and the developing nervous system (PMID: 36979146).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MACC1 antibody 21970-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Carcinog The role of metastasis-associated in colon cancer 1 (MACC1) in endometrial carcinoma tumorigenesis and progression. | ||
Int J Med Sci Bioinformatics Analysis Reveals a Novel Prognostic Model for Esophageal Squamous Cell Carcinoma |
