MAD1 Polyclonal antibody

MAD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18322-1-AP

Synonyms

hMAD1, HsMAD1, MAD1, MAD1 like protein 1, MAD1L1, PIG9, Tax binding protein 181, TP53I9, TXBP181



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, human brain tissue, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18322-1-AP targets MAD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAD1 fusion protein Ag13171
Full Name MAD1 mitotic arrest deficient-like 1 (yeast)
Calculated molecular weight 83 kDa
Observed molecular weight 83 kDa
GenBank accession numberBC009964
Gene symbol MAD1L1
Gene ID (NCBI) 8379
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].

Protocols

Product Specific Protocols
WB protocol for MAD1 antibody 18322-1-APDownload protocol
IHC protocol for MAD1 antibody 18322-1-APDownload protocol
IF protocol for MAD1 antibody 18322-1-APDownload protocol
IP protocol for MAD1 antibody 18322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Cycle

Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53.

Authors - Sara M Reed
humanWB

BMB Rep

TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction.

Authors - Suqin Shen
humanWB,IF

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan