MAD1 Polyclonal antibody

MAD1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 18322-1-AP

Synonyms

hMAD1, HsMAD1, MAD1, MAD1 like protein 1, MAD1L1, PIG9, Tax binding protein 181, TP53I9, TXBP181

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Validation Data Gallery

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  WB analysis using 18322-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18322-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 18322-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 18322-1-AP (MAD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18322-1-AP

  IP result of anti-MAD1 (IP:18322-1-AP, 5ug; Detection:18322-1-AP 1:1000) with HeLa cells lysate 880ug.

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  IHC staining of human brain using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18322-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18322-1-AP (MAD1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of A431 using 18322-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAD1 antibody (18322-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 18322-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 18322-1-AP (MAD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, human brain tissue, Raji cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 1 publications below

Product Information

18322-1-AP targets MAD1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAD1 fusion protein Ag13171
• Full Name: MAD1 mitotic arrest deficient-like 1 (yeast)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC009964
• Gene Symbol: MAD1L1
• Gene ID (NCBI): 8379
• RRID: AB_2139251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].

Publications

Species	Application	Title
human	WB,IF	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
mouse	WB	Cell Cycle; Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53.; Authors - Sara M Reed
human	WB	BMB Rep; TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction.; Authors - Suqin Shen
human	WB	Sci Adv; Biallelic germline mutations in MAD1L1 induce a syndrome of aneuploidy with high tumor susceptibility; Authors - Carolina Villarroya-Beltri
mouse,human	WB	Aging (Albany NY); The HNF4A-CHPF pathway promotes proliferation and invasion through interactions with MAD1L1 in glioma; Authors - Haitao Luo