|Positive WB detected in||A431 cells, HeLa cells, HepG2 cells, human brain tissue, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells, A431 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18322-1-AP targets MAD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAD1 fusion protein Ag13171|
|Full Name||MAD1 mitotic arrest deficient-like 1 (yeast)|
|Calculated molecular weight||83 kDa|
|Observed molecular weight||83 kDa|
|GenBank accession number||BC009964|
|Gene ID (NCBI)||8379|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MAD1L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. It recruits MAD2L1 to unattached kinetochores. Also it is required for anchoring MAD2L1 to the nuclear periphery. In addition, it binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding [PMID:10049595, 12837246].
|Product Specific Protocols|
|WB protocol for MAD1 antibody 18322-1-AP||Download protocol|
|IHC protocol for MAD1 antibody 18322-1-AP||Download protocol|
|IF protocol for MAD1 antibody 18322-1-AP||Download protocol|
|IP protocol for MAD1 antibody 18322-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nuclear interactor of ARF and Mdm2 regulates multiple pathways to activate p53.
TCP10L synergizes with MAD1 in transcriptional suppression and cell cycle arrest through mutual interaction.
Nucleic Acids Res
ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.