MAEA Polyclonal antibody

MAEA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15238-1-AP

Synonyms

EMLP, EMP, HLC 10, MAEA, PIG5

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Validation Data Gallery

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  WB analysis using 15238-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15238-1-AP (MAEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15238-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15238-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15238-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15238-1-AP (MAEA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells, MCF-7 cells, U2OS cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15238-1-AP targets MAEA in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAEA fusion protein Ag7365
• Full Name: macrophage erythroblast attacher
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC001225
• Gene Symbol: MAEA
• Gene ID (NCBI): 10296
• RRID: AB_2137505
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAEA(macrophage erythroblast attacher) is a core component of the CTLH E3 ubiquitin-protein ligase complex. MAEA is required for normal cell proliferation and plays a role in erythroblast enucleation during erythrocyte maturation and in the development of mature macrophages. N-terminus MAEA and full-length MAEA are mostly expressed in nuclear and can be detected at low level in the cytoplasm of some cells, while C-terminus MAEA can be detected in the cytoplasm and nucleoli within the nucleus. MAEA mediates the attachment of erythroid cell to mature macrophages which can inhibit erythroid cell apoptosis. The expression of MAEA may be associated with the type 2 diabetes mellitus.(PubMed: 28955747, 24143168, 29911972, 29911972, 30674470, 9763581).

Publications

Species	Application	Title
human	WB	iScience; Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24; Authors - Lalit R Patel