MAEA Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83267-1-RR (same clone as 83267-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83267-1-RR (MAEA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 83267-1-RR (same clone as 83267-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug MAEA Recombinant antibody (83267-1-RR, Clone:240097B2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83267-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00233-1

  Cytometric bead array standard curve ofMP00233-1, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-3-PBS. Standard: Ag27150. Range: 2.5-100 ng/mL.

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  Cytometric bead array standard curve of MP00233-2

  Cytometric bead array standard curve ofMP00233-2, MAEA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83267-1-PBS. Detection antibody: 83267-2-PBS. Standard: Ag27150. Range: 1.25-100 ng/mL.

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  Affinity and Kinetic Characterization of 83267-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83267-1-RR against Human MAEA were performed. The affinity constant is 0.496 nM.

MAEA Uni-rAb^TM Recombinant Antibody for Indirect ELISA, Cytometric bead array, WB

Cat No. 83267-1-PBS

Clone No.240097B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

Indirect ELISA, Cytometric bead array, WB

Synonyms

EMP, EMLP, EC:2.3.2.27, E3 ubiquitin-protein transferase MAEA, Cell proliferation-inducing gene 5 protein

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Product Information

83267-1-PBS targets MAEA as part of a matched antibody pair:

MP00233-1: 83267-1-PBS capture and 83267-3-PBS detection (validated in Cytometric bead array)

MP00233-2: 83267-1-PBS capture and 83267-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D<1.00 x 10^-12M
  K_Off<1.00 x 10^-6M
  K_On=9.49 x 10^4M
• Immunogen: MAEA fusion protein Ag27150
• Full Name: macrophage erythroblast attacher
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC001225
• Gene Symbol: MAEA
• Gene ID (NCBI): 10296
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7L5Y9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.