MAF1 Polyclonal antibody

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• MAF1 Polyclonal antibody (11619-1-AP)

MAF1 Polyclonal Antibody for WB, ELISA

Cat No. 11619-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MAF 1

• 

  WB analysis using 11619-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11619-1-AP (MAF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, J774A.1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11619-1-AP targets MAF1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2198

  Product name: Recombinant human MAF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-256 aa of BC018714

  Sequence: MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGAEETSTMEEDRVPVICI

• Full Name: MAF1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 256 aa, 29 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC018714
• Gene Symbol: MAF1
• Gene ID (NCBI): 84232
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H063
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAF1 operates as a conserved repressor of RNA polymerase III transcription, first identified in yeast. It binds RNA pol III directly, blocking TFIIIB recruitment and dampening tRNA and 5S rRNA output. In mammals, MAF1 additionally fine-tunes select RNA pol II genes, impacting metabolism, bone formation, adipogenesis, and oncogenesis, while its own activity is stringently controlled by mTORC1 phosphorylation.