MAFF Recombinant monoclonal antibody

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  WB analysis using 85186-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85186-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 85186-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 85186-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 85186-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 85186-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MAFF Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85186-3-RR

Clone No.242859F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242859F9, U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

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Tested Applications

• Positive WB detected in: K-562 cells, mouse placenta tissue, HuH-7 cells, L02 cells, 4T1 cells
• Positive IHC detected in: human lung cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85186-3-RR targets MAFF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3460

  Product name: Recombinant human MAFF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-164 aa of BC015037

  Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS

• Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
• Calculated Molecular Weight: 17 aa, 8 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: BC015037
• Gene Symbol: MAFF
• Gene ID (NCBI): 23764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9ULX9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).