MAFK Polyclonal antibody, PBS Only

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• MAFK Polyclonal antibody, PBS Only (33199-1-PBS)

• 

  WB analysis using 33199-1-AP (same clone as 33199-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33199-1-AP (MAFK antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 33199-1-AP (same clone as 33199-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAFK antibody (33199-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33199-1-PBS in a different storage buffer formulation.

MAFK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 33199-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Erythroid transcription factor NF-E2 p18 subunit, Transcription factor MafK

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Product Information

33199-1-PBS targets MAFK in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag38230

  Product name: Recombinant human MAFK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-156 aa of NM_002360.3

  Sequence: MTTNPKPNKALKVKKEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQRRRTLKNRGYAASCRIKRVTQKEELERQRVELQQEVEKLARENSSMRLELDALRSKYEALQTFARTVARGPVAPSKVATTSVITIVKSTELSSTSVPFSAAS

• Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)
• Calculated Molecular Weight: 18 kDa，156aa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: NM_002360.3
• Gene Symbol: MAFK
• Gene ID (NCBI): 7975
• RRID: AB_3742891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O60675
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The small Maf proteins (sMafs) are basic region leucine zipper (bZIP)-type transcription factors. MAFF, MAFG, and MAFK are the three vertebrate sMafs (PMID: 27058431). They act as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2, and NFE2L3/NRF3, and recruiting them to specific DNA-binding sites (PMID: 8932385; 9150357). In cancer cell lines, MAFK promotes epithelial-to-mesenchymal transition (EMT) by downregulating E-cadherin expression through the targeting of transmembrane glycoprotein nonmetastatic B (GPNMB), a potent inducer of EMT (PMID: 28400538; 36061142).