MAG Monoclonal antibody

MAG Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66709-1-Ig
Clone No.3H9G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

GMA, MAG, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66709-1-Ig targets MAG in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6065

Product name: Recombinant human MAG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 199-516 aa of BC053347

Sequence: VSLLHFVPTREANGHRLGCQASFPNTTLQFEGYASMDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGTVLREAVAESLLLELEEVTPAEDGVYACLAENAYGQDNRTVGLSVMYAPWKPTVNGTMVAVEGETVSILCSTQSNPDPILTIFKEKQILSTVIYESELQLELPAVSPEDDGEYWCVAENQYGQRATAFNLSVEFAPVLLLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNESEREFVYSERSGLVLTSILTLRGQAQAPPRVICTARNLYGAKSLELPFQGAHRLMWAKIGP

Predict reactive species
Full Name myelin associated glycoprotein
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC053347
Gene Symbol MAG
Gene ID (NCBI) 4099
RRIDAB_2882061
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP20916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.

Protocols

Product Specific Protocols
WB protocol for MAG antibody 66709-1-IgDownload protocol
IHC protocol for MAG antibody 66709-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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