MAG Recombinant antibody, PBS Only (Detector)

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  WB analysis of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86097-1-RR (MAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02467-1

  Sandwich ELISA standard curve of MP02467-1, Human MAG Recombinant Matched Antibody Pair - PBS only. 86097-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2975. 86097-1-PBS was HRP conjugated as the detection antibody. Range: 46.9-3000 pg/mL.

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  Sample test of MP02467-1

  The mean MAG concentration was determined to be 1.2 ng/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

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  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86097-1-RR (same clone as 86097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86097-1-RR (MAG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86097-1-PBS in a different storage buffer formulation.

MAG Uni-rAb^TM Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86097-1-PBS

Clone No.250555A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

GMA, myelin associated glycoprotein, S MAG, SIGLEC 4A, SIGLEC4A

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Product Information

86097-1-PBS targets MAG as part of a matched antibody pair:

MP02467-1: 86097-2-PBS capture and 86097-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAG fusion protein Eg2975
• Full Name: myelin associated glycoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_002361.3
• Gene Symbol: MAG
• Gene ID (NCBI): 4099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20916-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAG (myelin associated glycoprotein) is a 100-kDa transmembrane glycoprotein that is a member of the SIGLEC family of proteins. MAG is localized in periaxonal Schwann cell and oligodendroglial membranes of myelin sheaths where it functions in glia-axon interactions (PMID: 17241126). It is a functional ligand for the Nogo-66 receptor (PMID: 12089450). MAG is a cell adhesion molecule for postnatal neural development and is thought to be involved in the process of myelination.