Rat MAG/Siglec-4a Recombinant monoclonal antibody, PBS Only

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  WB analysis of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 86073-3-RR (same clone as 86073-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86073-3-RR (same clone as 86073-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86073-3-RR (MAG/Siglec-4a antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAG/Siglec-4a antibody (86073-3-RR, Clone: 250740E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

• 

  IF Staining of rat brain using 86073-3-RR (same clone as 86073-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using MAG/Siglec-4a antibody (86073-3-RR, Clone: 250740E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86073-3-PBS in a different storage buffer formulation.

MAG/Siglec-4a Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86073-3-PBS

Clone No.250740E3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

1B236, Brain neuron cytoplasmic protein 3, Mag, Myelin-associated glycoprotein, Sialic acid-binding Ig-like lectin 4a

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Product Information

86073-3-PBS targets MAG/Siglec-4a in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3251

  Product name: Recombinant Rat MAG protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-516 aa of NM_017190.4

  Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

• Full Name: myelin-associated glycoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-100 kDa
• GenBank Accession Number: NM_017190.4
• Gene Symbol: Mag
• Gene ID (NCBI): 29409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07722-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.