Tested Applications
| Positive WB detected in | A375 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12233-1-AP targets MAGEA1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2871 Product name: Recombinant human MAGEA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC017555 Sequence: MSLEQRSLHCKPEEALEAQQEALGLVCVQAAASSSSPLVLGTLEEVPTAGSTDPPQSPQGASAFPTTINFTRQRQPSEGSSSREEEGPSTSCILESLFRAVITKKVADLVGFLLLKYRAREPVTKAEMLESVIKNYKHCFPEIFGKASESLQLVFGIDVKEADPTGHSYVLVTCLGLSYDGLLGDNQIMPKTGFLIIVLVMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV Predict reactive species |
| Full Name | melanoma antigen family A, 1 (directs expression of antigen MZ2-E) |
| Calculated Molecular Weight | 309 aa, 34 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC017555 |
| Gene Symbol | MAGEA1 |
| Gene ID (NCBI) | 4100 |
| RRID | AB_2250080 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43355 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAGEA1 antibody 12233-1-AP | Download protocol |
| WB protocol for MAGEA1 antibody 12233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAGEA1 Polyclonal antibody (Cat# 12233-1-AP) has 1 citation to date. It appears in the Journal of Molecular Histology, where it was used for Western blot in a rat study. The research focuses on the role of MAGEA1 in cryptorchidism and its associated transcription factor regulatory network, placing it within the fields of molecular histology, reproductive biology, and gene regulation.
| Species | Application | Title |
|---|---|---|
J Mol Histol Comprehensively exploring the roles of MAGEA1 in cryptorchidism and its related transcription factor regulatory network. |
