Filter: AllWBIHC

WB analysis of mouse brain using 60054-1-Ig (same clone as 60054-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 60054-1-Ig (same clone as 60054-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 60054-1-Ig (same clone as 60054-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 60054-1-Ig (same clone as 60054-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 60054-1-Ig (same clone as 60054-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 60054-1-Ig (same clone as 60054-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human oesophagus cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

MAGEA3 Monoclonal antibody, PBS Only

Name: MAGEA3 Monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 60054-1-PBS

MAGEA3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60054-1-PBS

Clone No.1A9F3

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

1A9F3, Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

60054-1-PBS targets MAGEA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0265 Product name: Recombinant human MAGEA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC000340 Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLE Predict reactive species
Full Name	melanoma antigen family A, 3
Calculated Molecular Weight	314 aa, 35 kDa
Observed Molecular Weight	45-48 kDa
GenBank Accession Number	BC000340
Gene Symbol	MAGEA3
Gene ID (NCBI)	4102
RRID	AB_2138124
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P43357
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.