MAGEA3 Monoclonal antibody, PBS Only

• 

  WB analysis of mouse brain using 60054-1-Ig (same clone as 60054-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60054-1-Ig (same clone as 60054-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 60054-1-Ig (same clone as 60054-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 60054-1-Ig (same clone as 60054-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60054-1-Ig (same clone as 60054-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 60054-1-Ig (same clone as 60054-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:25 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60054-1-Ig(MAGEA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60054-1-Ig (same clone as 60054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60054-1-Ig (MAGEA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60054-1-PBS in a different storage buffer formulation.

MAGEA3 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60054-1-PBS

Clone No.1A9F3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

Antigen MZ2 D, Cancer/testis antigen 1.3, CT1.3, HIP8, HYPD, MAGE 3 antigen, MAGE3, MAGEA3, MAGE-A3, MAGEA6, melanoma antigen family A, 3, Melanoma associated antigen 3

---

Product Information

60054-1-PBS targets MAGEA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0265

  Product name: Recombinant human MAGEA3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-229 aa of BC000340

  Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAGLLIIVLAIIAREGDCAPEEKIWEELSVLE

• Full Name: melanoma antigen family A, 3
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35-55 kDa
• GenBank Accession Number: BC000340
• Gene Symbol: MAGEA3
• Gene ID (NCBI): 4102
• RRID: AB_2138124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P43357
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.