MAGEB3 Polyclonal antibody, PBS Only

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• MAGEB3 Polyclonal antibody, PBS Only (18000-1-PBS)

MAGEB3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 18000-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

• 

  WB analysis using 18000-1-AP (same clone as 18000-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18000-1-AP (MAGEB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18000-1-AP (same clone as 18000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 18000-1-AP (same clone as 18000-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18000-1-AP (MAGEB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18000-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

18000-1-PBS targets MAGEB3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12476

  Product name: Recombinant human MAGEB3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-346 aa of BC074756

  Sequence: MPRGQKSTLHAREKRQQTRGQTQDHQGAQITATNKKKVSFSSPLILGATIQKKSAGRSRSALKKPQRALSTTTSVDVSYKKSYKGANSKIEKKQSFSQGLSSTVQSHTDPLTMKTNMLVQFLMEMYKMKKPIMKADMLKIVQKSHKNCFPEILKKASFNMEVVFGVDLKKVDSTKDSYVLVSKMDLPNNGTVTRGRGFPKTGLLLNLLGVIFMKGNCATEEKIWEFLNKMRIYDGKKHFIFGEPRKLITQDLVKLKYLEYRQVPNSNPARYEFLWGPRAHAETSKMKVLEFWAKVNKTVPSAFQFWYEEALRDEEERVQAAAMLNDGSSAMGRKCSKAKASSSSHA

• Full Name: melanoma antigen family B, 3
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC074756
• Gene Symbol: MAGEB3
• Gene ID (NCBI): 4114
• RRID: AB_3085548
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15480
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types and Testis/placenta, but not in other normal tissues. MAGEB3, also named CT3.5, is a member of the MAGEB gene family.