MAGEC3 Antibody

MAGEC3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21491-1-AP

Synonyms

Cancer/testis antigen 7.2, CT7.2, HCA2, MAGE C3, MAGE C3 antigen, MAGEC3, MAGEC4, melanoma antigen family C, 3, Melanoma associated antigen C3



Tested Applications

Positive WB detected inA375 cells, mouse thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The 21491-1-AP immunogen is Fusion Protein MAGEC3 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEC3 fusion protein Ag15819
Full Name melanoma antigen family C, 3
Calculated molecular weight 643aa,72 kDa
Observed molecular weight 70-72 kDa
GenBank accession number BC103893
Gene symbol MAGEC3
Gene ID (NCBI) 139081
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEC3 antibody 21491-1-APDownload protocol
IHC protocol for MAGEC3 antibody 21491-1-APDownload protocol
IF protocol for MAGEC3 antibody 21491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

BMC Cancer

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells.

Authors - Xiao Song