MAGED4 Polyclonal antibody

MAGED4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16809-1-AP

Synonyms

KIAA1859, MAGE D4 antigen, MAGE E1, MAGE E1 antigen, MAGE1, MAGED4, MAGED4A, MAGED4B, MAGEE1, melanoma antigen family D, 4, Melanoma associated antigen D4

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Validation Data Gallery

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  WB analysis of Jurkat using 16809-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16809-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 16809-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells
• Positive IHC detected in: human gliomas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16809-1-AP targets MAGED4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGED4 fusion protein Ag10329
• Full Name: melanoma antigen family D, 4
• Calculated Molecular Weight: 741 aa, 81 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC081566
• Gene Symbol: MAGED4
• Gene ID (NCBI): 728239
• RRID: AB_2138678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).