MAK16 Polyclonal antibody

MAK16 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 17505-1-AP

Synonyms

MAK16, MAK16 homolog (S. cerevisiae), MAK16L, NNP78, Protein MAK16 homolog, Protein RBM13, RBM13

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Validation Data Gallery

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  WB analysis using 17505-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17505-1-AP (MAK16 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 17505-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 17505-1-AP (MAK16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17505-1-AP targets MAK16 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAK16 fusion protein Ag11705
• Full Name: MAK16 homolog (S. cerevisiae)
• Calculated Molecular Weight: 300 aa, 35 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC050528
• Gene Symbol: MAK16
• Gene ID (NCBI): 84549
• RRID: AB_2281504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.