MALT1 Monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66225-1-Ig (same clone as 66225-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66225-1-Ig (same clone as 66225-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66225-1-Ig (MALT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66225-1-Ig (MALT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66225-1-Ig(MALT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66225-1-Ig (same clone as 66225-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MALT1 Monoclonal antibody (66225-1-Ig, Clone:4D3F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66225-1-PBS in a different storage buffer formulation.

MALT1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66225-1-PBS

Clone No.4D3F6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

4D3F6, EC:3.4.22.-, MALT 1, MALT lymphoma-associated translocation, MLT

---

Product Information

66225-1-PBS targets MALT1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20632

  Product name: Recombinant human MALT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 126-450 aa of BC030143

  Sequence: GIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRSVDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIDELNNLGHPDNKEQTTDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLL

• Full Name: mucosa associated lymphoid tissue lymphoma translocation gene 1
• Calculated Molecular Weight: 824 aa, 92 kDa
• Observed Molecular Weight: 92-100 kDa
• GenBank Accession Number: BC030143
• Gene Symbol: MALT1
• Gene ID (NCBI): 10892
• RRID: AB_2881616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UDY8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mucosa-associated lymphoid tissue lymphoma translocation protein 1 belongs to caspase-like protein superfamily.MALT1 plays a potential role in the regulation of apoptosis and induces NFKB activation and required for the normal development of B cells and for the activation of the IKK complex.It acts as a caspase and mediates additional functions related to T cell activation, cleaving both the ubiquitin editing enzyme,TNFAIP3,and the C terminus of BCL10 .It has two isoforms.Researches showed that inhibition of MALT1 blocks A20 and BCL10 cleavage, reduces NFKB activity, and decreases the expression of NFKB targets BCLXL.