MAML1 Polyclonal antibody

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  WB analysis using 55493-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55493-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55493-1-AP (MAML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

MAML1 Polyclonal Antibody for WB, ELISA

Cat No. 55493-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Synonyms

KIAA0200, Mam 1, Mam1, MAML1, mastermind like 1 (Drosophila), Mastermind like protein 1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• CoIP: See 1 publications below

Product Information

55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mastermind-like 1 (Drosophila)
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108-120 kDa
• GenBank Accession Number: NM_014757
• Gene Symbol: MAML1
• Gene ID (NCBI): 9794
• RRID: AB_11182286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92585
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Immunol; Identification of SCAMP2 as a regulator of NOTCH signaling in cisplatin resistance through a novel prognostic model for bladder cancer; Authors - Longjun Cai
human	WB,CoIP	Int J Biol Sci; Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.; Authors - Yanzhu Shi
human	WB	Discov Oncol; CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway; Authors - Hairong Zhou