Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55493-1-AP targets MAML1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | mastermind-like 1 (Drosophila) |
| Calculated Molecular Weight | 108 kDa |
| Observed Molecular Weight | 108-120 kDa |
| GenBank Accession Number | NM_014757 |
| Gene Symbol | MAML1 |
| Gene ID (NCBI) | 9794 |
| RRID | AB_11182286 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92585 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MAML1 antibody 55493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAML1 Polyclonal antibody (Cat# 55493-1-AP) has 5 total citations. These appear in Circulation, International Journal of Biological Sciences, British Journal of Cancer, Frontiers in Immunology, and Discover Oncology. The associated research fields span Notch signaling pathway regulation, congenital heart disease, and multiple cancer types including breast, lung, bladder, and colorectal cancers, with a focus on metastasis, chemoresistance, and epigenetic mechanisms.
| Species | Application | Title |
|---|---|---|
Circulation Aberrant Phase Separation of Endothelial MAML1 Causes Congenital Heart Disease by Suppressing Notch Activity | ||
Int J Biol Sci Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling. | ||
Br J Cancer RNLS hypomethylation as a potential epigenetic marker of lung cancer metastasis: an epigenome-wide association study. | ||
Front Immunol Identification of SCAMP2 as a regulator of NOTCH signaling in cisplatin resistance through a novel prognostic model for bladder cancer | ||
Discov Oncol CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway |
