MAN1A2 Polyclonal antibody

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  WB analysis of human placenta using 14049-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14049-1-AP (MAN1A2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14049-1-AP

  IP result of anti-MAN1A2 (IP:14049-1-AP, 4ug; Detection:14049-1-AP 1:200) with human placenta tissue lysate 4000ug.

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  IHC staining of human placenta using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 14049-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14049-1-AP (MAN1A2 antibody) at dilution of 1:50 (under 40x lens).

MAN1A2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14049-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

Alpha 1,2 mannosidase IB, MAN1A2, MAN1B

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Tested Applications

• Positive WB detected in: human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human placenta tissue, human heart tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14049-1-AP targets MAN1A2 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5240

  Product name: Recombinant human MAN1A2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 292-641 aa of BC063300

  Sequence: VQLAEKLLPAFNTPTGIPWAMVNLKSGVGRNWGWASAGSSILAEFGTLHMEFIHLSYLTGDLTYYKKVMHIRKLLQKMDRPNGLYPNYLNPRTGRWGQYHTSVGGLGDSFYEYLLKAWLMSDKTDHEARKMYDDAIEAIEKHLIKKSRGGLTFIGEWKNGHLEKKMGHLACFAGGMFALGADGSRADKAGHYLELGAEIARTCHESYDRTALKLGPESFKFDGAVEAVAVRQAEKYYILRPEVIETYWYLWRFTHDPRYRQWGWEAALAIEKYCRVNGGFSGVKDVYSSTPTHDDVQQSFFLAETLKYLYLLFSGDDLLPLDHWVFNTEAHPLPVLHLANTTLSGNPAVR

• Full Name: mannosidase, alpha, class 1A, member 2
• Calculated Molecular Weight: 641 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC063300
• Gene Symbol: MAN1A2
• Gene ID (NCBI): 10905
• RRID: AB_2266033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60476
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first group or Class I mannosidase is comprised of α1-2 mannosidases from mammals and yeast with encoded polypeptides of 65-75 kDa. Most of them are Ca2+-dependent enzymes located in the ER and/or Golgi which are inhibited by substrate mimics like kifunensine and deoxymannojirimycin. Their specificity is restricted to α(1-2) linkages, and they are mostly inactive towards artificial substrates like arylmannosides(PMID:10207175).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	World J Gastroenterol; Hepatitis B virus upregulates host expression of α-1,2-mannosidases via the PPARα pathway.; Authors - Song Hu