MAOB Polyclonal antibody

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• MAOB Polyclonal antibody (12602-1-AP)

MAOB Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12602-1-AP Copied!

Publications (34)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Amine oxidase [flavin-containing] B, EC:1.4.3.21, MAO B, MAO-B, monoamine oxidase B

• 

  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 12602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 12602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 12602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 12602-1-AP

  IP result of anti-MAOB (IP:12602-1-AP, 4ug; Detection:12602-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 12602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12602-1-AP (MAOB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 12602-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12602-1-AP (MAOB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: L02 cells, mouse liver tissue, human liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12602-1-AP targets MAOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3253

  Product name: Recombinant human MAOB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC022494

  Sequence: MSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDHVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIQSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDT

• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• RRID: AB_2137273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27338
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).

Publications

What published studies show

The Rabbit anti-MAOB polyclonal antibody (Cat# 12602-1-AP) has accumulated 34 citations published in journals including Science, Cell, Nature Neuroscience, Neuron, Advanced Science, Cell Communication and Signaling, EMBO Reports, Journal of Neuroinflammation, and Experimental Neurology. Associated research fields span Parkinson's disease, neurotransmitter metabolism, neuroinflammation, gut-brain axis interactions, cancer (gastric, lung, prostate), ferroptosis, atherosclerosis, epilepsy, and depression.

Automatically generated from the 34 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Science; Induction of astrocytic Slc22a3 regulates sensory processing through histone serotonylation; Authors - Debosmita Sardar
mouse	WB	Cell; A skin-hypothalamus axis couples heat stress and metabolic dysfunction.; Authors - Hai-Yan Zhou
mouse	IF	Nat Neurosci; GABA-dependent microglial elimination of inhibitory synapses underlies neuronal hyperexcitability in epilepsy; Authors - Zhang-Peng Chen
		Neuron; Astrocytic NFIX regulates thalamocortical circuits through GABA and purinergic signaling.; Authors - Sanjana Murali
mouse	IF	Neuron; Social deprivation induces astrocytic TRPA1-GABA suppression of hippocampal circuits; Authors - Yi-Ting Cheng
mouse	WB,IF	Adv Sci (Weinh); Neuroprotective Effects of Time-Restricted Feeding Combined With Different Protein Sources in MPTP-Induced Parkinson's Disease Mice Model and Its Modulatory Impact on Gut Microbiota Metabolism.; Authors - Ting Li