MAOB Polyclonal antibody

MAOB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 12602-1-AP

Synonyms

MAO B, MAOB, Monoamine Oxidase B, Monoamine oxidase type B

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Validation Data Gallery

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  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12602-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12602-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12602-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12602-1-AP (MAOB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12602-1-AP

  IP result of anti-MAOB (IP:12602-1-AP, 4ug; Detection:12602-1-AP 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human gliomas using 12602-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12602-1-AP (MAOB antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: L02 cells, mouse liver tissue, human liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 6 publications below

Product Information

12602-1-AP targets MAOB in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAOB fusion protein Ag3253
• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• RRID: AB_2137273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position (PMID:3387449).

Publications

Species	Application	Title
mouse	IF	Neuron; Social deprivation induces astrocytic TRPA1-GABA suppression of hippocampal circuits; Authors - Yi-Ting Cheng
mouse	IF	EMBO Rep; Regional heterogeneity of astrocyte morphogenesis dictated by the formin protein, Daam2, modifies circuit function.; Authors - Juyeon Jo
mouse	IHC	J Transl Med; Identification and experimental validation of KMO as a critical immune-associated mitochondrial gene in unstable atherosclerotic plaque; Authors - Fu-Jun Liao
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang