Tested Applications
| Positive WB detected in | HeLa cells, Chloroquine treated HeLa cells |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells, HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18725-1-AP targets LC3B-Specific in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | microtubule-associated protein 1 light chain 3 beta |
| Calculated Molecular Weight | 15 kDa |
| Observed Molecular Weight | 15 kDa, 18 kDa |
| GenBank Accession Number | NM_022818 |
| Gene Symbol | LC3B |
| Gene ID (NCBI) | 81631 |
| ENSEMBL Gene ID | ENSG00000140941 |
| RRID | AB_2137745 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IF protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| IHC protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| WB protocol for LC3B-Specific antibody 18725-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LC3B-Specific Polyclonal antibody (Cat# 18725-1-AP) has 495 total citations. These appear in high-impact journals including Nature, Cell Research, Autophagy, Nature Neuroscience, Nature Communications, Cell Death & Disease, Biomaterials, ACS Nano, and Advanced Science. Research fields span autophagy and mitophagy, ferroptosis, cancer biology, neurodegeneration (Alzheimer's, Parkinson's, Huntington's), diabetes, cardiovascular disease, and inflammation, with applications mainly in Western blot, immunofluorescence, and IHC.
| Species | Application | Title |
|---|---|---|
Cell Res Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models. | ||
J Extracell Vesicles Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles. | ||
Am J Respir Crit Care Med Acid Sphingomyelinase Inhibition Attenuates Cell Death in Mechanically-Ventilated Newborn Rat Lung. | ||
Nat Neurosci The mitochondrial unfolded protein response in human microglia disrupts neuronal-glial communication and promotes senescence. | ||
Reviews
What customers say
Overall, users report mixed results for this LC3B antibody in Western blot. Two reviewers noted strong non-specific bands above 20 kDa and weak target signal, particularly in extracellular vesicles where detection was nearly absent. In cell lysates, signal was only slightly detectable at 1:500. However, other users found it effective in mouse brain and NSC34 cells at 1:300–1:500, with one also reporting strong IHC labeling in primary rat cortical neurons. Performance in iPSC-derived microglia with bafilomycin treatment was satisfactory. Results appear sample-dependent, with some applications yielding clear signals while others show background issues.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jacob (Verified Customer) (07-23-2026) | Has a very strong non-specific banding pattern above 20kDa, so the LC3B was faint. 2*
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Alicia (Verified Customer) (09-30-2025) | Control microglia derived from iPSC with and without bafilomycin. Drug treated microglia derived from iPSC with and without bafilomycin.
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Allegra (Verified Customer) (09-30-2025) | Work for cell lysates only in concentration 1:500 and this is only slightly detectable. In extracellular vesicles nearly no signal.
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Azita (Verified Customer) (06-02-2021) | Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.
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Tanusree (Verified Customer) (12-03-2019) | The antibody works great in Western blotting analysis.
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