|Positive WB detected in||human brain tissue, MCF-7 cells, TN treated Hela, A549 cells, UV treated HEK-293, mouse brain tissue, HepG2 cells|
|Positive IHC detected in||mouse brain tissue, rat brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Chloroquine treated HepG2 cells, Starvation treated HEK-293 cells, Starvation treated HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:300-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18725-1-AP targets LC3B-Specific in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||microtubule-associated protein 1 light chain 3 beta|
|Calculated molecular weight||15 kDa|
|Observed molecular weight||15 kDa, 18 kDa|
|GenBank accession number||NM_022818|
|Gene ID (NCBI)||81631|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.
|Product Specific Protocols|
|WB protocol for LC3B-Specific antibody 18725-1-AP||Download protocol|
|IHC protocol for LC3B-Specific antibody 18725-1-AP||Download protocol|
|IF protocol for LC3B-Specific antibody 18725-1-AP||Download protocol|
|FC protocol for LC3B-Specific antibody 18725-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Exp Cell Res
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J Immunol Res
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-02-2021)
Western blot analysis using LC3B-Specific Polyclonal antibody in NSC34 cell line at dilution of 1:300. Immunohistochemistry labelling of primary rat cortical neurons using LC3B-Specific Polyclonal antibody at dilution of 1:500 showed strong labelling.
Tanusree (Verified Customer) (12-03-2019)
The antibody works great in Western blotting analysis.