LC3B-Specific Antibody 28 Publications

Rabbit Polyclonal| Catalog number: 18725-1-AP

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Freight/Packing

Con: 45 μg/150 μl

Choose size:

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Species specificity:
human, mouse, rat

Positive WB detected in:
Chloroquine treated HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF-7 cells, mouse brain tissue, rat brain tissue

Positive IHC detected in:
human heart tissue, human heart tissue, human testis tissue

Positive IF detected in:
Chloroquine treated HepG2 cells, HEK-293 cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
microtubule-associated protein 1 light chain 3 beta

Calculated molecular weight:
15 kDa

Observed molecular weight:
15kDa, 18 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
MAP1LC3B

Synonyms
LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3, MAP1LC3B
Background

LC3B, also named as MAP1LC3B, MAP1A/1BLC3, belongs to the MAP1 LC3 family. It is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. In cell biology, autophagy, or autophagocytosis, is a catabolic process involving the degradation of a cell's own components through the lysosomalmachinery. It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Two forms of LC3, called LC3-I (17-19kd) and -II(14-16kd), were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. The amount of LC3-II is correlated with the extent of autophagosome formation. LC3-II is the first mammalian protein identified that specifically associates with autophagosome membranes. MAP1LC3 has 3 isoforms MAP1LC3A, MAP1LC3B and MAP1LC3C. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, like their rat counterpart, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form. This antibody is specific to LC3B.


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