MEKK2 Polyclonal antibody

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  WB analysis using 55106-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55106-1-AP (MEKK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 55106-1-AP

  IP result of anti-MEKK2 (IP:55106-1-AP, 4ug; Detection:55106-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 55106-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 55106-1-AP (MEKK2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MEKK2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 55106-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MAP3K2, EC:2.7.11.25, MEKK2B, MEKK 2, MEK kinase 2

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

55106-1-AP targets MEKK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  fusion protein

• Full Name: mitogen-activated protein kinase kinase kinase 2
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: NM_006609
• Gene Symbol: MAP3K2
• Gene ID (NCBI): 10746
• RRID: AB_11064604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2U5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K2, also named as MEKK2 and MEKK2B, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and MAPK kinase kinase subfamily. It is a component of a protein kinase signal transduction cascade. MAP3K2 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. The antibody is specific to MAP3K2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IP,WB	Mol Cancer; A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer; Authors - Runjie Song
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu