ASK1 Polyclonal antibody

ASK1 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14385-1-AP

Synonyms

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

14385-1-AP targets ASK1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, monkey, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASK1 fusion protein Ag5818
Full Name mitogen-activated protein kinase kinase kinase 5
Calculated molecular weight 155 kDa
GenBank accession numberBC054503
Gene symbol MAP3K5
Gene ID (NCBI) 4217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38.

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Thioredoxin-1 phosphorylated at T100 is needed for its anti-apoptotic activity in HepG2 cancer cells.

Authors - Chen Xiaoping X
monkeyWB

Cell Microbiol

\Non-haemolytic enterotoxin (Nhe) of Bacillus cereus induces apoptosis in Vero cells.

Authors - Xiaoye Liu
ratWB

J Mol Cell Cardiol

Enhanced MiR-711 transcription by PPARγ induces endoplasmic reticulum stress-mediated apoptosis targeting calnexin in rat cardiomyocytes after myocardial infarction.

Authors - Na Zhao