Tested Applications
| Positive IHC detected in | human liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16212-1-AP targets MAP3K6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9206 Product name: Recombinant human MAP3K6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC015914 Sequence: MNLLLSYRDVQDYSAIIELVETLQALPTCDVAEQHNVCFHYTFALNRRNRPGDRAKALSVLLPLVQLEGSVAPDLYCMCGRIYKDMFFSSGFQDAGHREQAYHWYRKAFDVEPSLHSGINAAVLLIAAGQHFEDSKELRLIGMKLGCLLARKGCVEKMQYYWDVGFYLGAQILANDPTQVVLAAEQLYKLNAPIWYLVSVMETFLLYQHFRPTPEPPGGPPRRAHFWLHFLLQSCQPFKTACAQGDQCLVLVLEMNKVLLPAKLEVRGTDPVSTVTLSLLEPETQDIPSSWTFPVASICGVSASKRD Predict reactive species |
| Full Name | mitogen-activated protein kinase kinase kinase 6 |
| Calculated Molecular Weight | 1148aa,128 kDa; 1011aa,113 kDa; 797aa,89 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC015914 |
| Gene Symbol | MAP3K6 |
| Gene ID (NCBI) | 9064 |
| RRID | AB_2878229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95382 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAP3K6 antibody 16212-1-AP | Download protocol |
| IHC protocol for MAP3K6 antibody 16212-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAP3K6 Polyclonal antibody (Cat# 16212-1-AP) has 1 total citation. It was cited in the European Journal of Medical Research (Impact Factor 4.8), in a study investigating the mechanism of ultrasound-guided renal parenchymal injection of mesenchymal stem cells for treating chronic kidney disease, specifically examining how down-regulated SGK1 promotes M2 macrophage polarization. The research field encompasses nephrology, stem cell therapy, and immune cell polarization. The antibody was used for Western blot in rat samples.
| Species | Application | Title |
|---|---|---|
Eur J Med Res Mechanism of ultrasound-guided renal parenchymal injection of MSCs for the treatment of chronic kidney disease: down-regulated SGK1 promotes M2 macrophage polarization. |
