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TAK1 Polyclonal antibody

TAK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29167-1-AP

Synonyms

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TAK1 Polyclonal antibody (29167-1-AP)

    WB analysis using 29167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29167-1-AP (TAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TAK1 Polyclonal antibody (29167-1-AP)

    IHC staining of human prostate cancer using 29167-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29167-1-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29167-1-AP targets TAK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TAK1 fusion protein Ag30725
Full Name mitogen-activated protein kinase kinase kinase 7
Calculated Molecular Weight 579 aa, 64 kDa
Observed Molecular Weight64-70 kDa
GenBank Accession NumberBC017715
Gene Symbol MAP3K7
Gene ID (NCBI) 6885
RRIDAB_2918242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TAK1 antibody 29167-1-APDownload protocol
IHC protocol for TAK1 antibody 29167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Manfred (Verified Customer) (05-30-2022)

This antibody worked perfectly in 1:1000 dilution (5% milk in PBST). I preferred to do overnight incubation at 4 degrees.

  • Applications: Western Blot
  • Cell Tissue Type: PBMCs