TAB2 Polyclonal antibody

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• KD/KO Validated

TAB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14410-1-AP Copied!

Publications (28)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MAP3K7IP2, KIAA0733, Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAB 2, TAB-2

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  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14410-1-AP

  WB result of TAB2 antibody (14410-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAB2 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of L02 using 14410-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14410-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 14410-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14410-1-AP (TAB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 14410-1-AP

  IP result of anti-TAB2 (IP:14410-1-AP, 4ug; Detection:14410-1-AP 1:600) with HepG2 cells lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human malignant melanoma using 14410-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14410-1-AP (TAB2 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14410-1-AP

  Immunofluorescent analysis of HepG2 cells, using MAP3K7IP2 antibody 14410-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: L02 cells, C6 cells, HepG2 cells, human liver tissue, Raji cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14410-1-AP targets TAB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5742

  Product name: Recombinant human MAP3K7IP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 344-693 aa of BC035910

  Sequence: SGPRTSSTSSSVNSQTLNRNQPTVYIAASPPNTDELMSRSQPKVYISANAATGDEQVMRNQPTLFISTNSGASAASRNMSGQVSMGPAFIHHHPPKSRAIGNNSATSPRVVVTQPNTKYTFKITVSPNKPPAVSPGVVSPTFELTNLLNHPDHYVETENIQHLTDPTLAHVDRISETRKLSMGSDDAAYTQALLVHQKARMERLQRELEIQKKKLDKLKSEVNEMENNLTRRRLKRSNSISQIPSLEEMQQLRSCNRQLQIDIDCLTKEIDLFQARGPHFNPSAIHNFYDNIGFVGPVPPKPKDQRSIIKTPKTQDTEDDEGAQWNCTACTFLNHPALIRCEQCEMPRHF

• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 2
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC035910
• Gene Symbol: MAP3K7IP2
• Gene ID (NCBI): 23118
• RRID: AB_2281638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NYJ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tab2 has a role in the inflammatory signal transduction pathway and is involved in the reversal of NCoR-dependent and NFkB- or APP-mediated gene repression and androgen antagonist response. Besides, it is identified as a cofactor for ERBB4 to assemble NCoR corepressor complex in response to neuregulin [PMID:16469706]. It also functions as an adaptor that links TAK1 and TRAF6 in response to interleukin-1β (IL-1β) or tumour necrosis factor-α (TNF-α), and thereby mediates TAK1 activation [PMID:11460167].

Publications

What published studies show

TAB2 antibody (Cat# 14410-1-AP) has 28 citations across journals including Nature Communications, Molecular Cell, Advanced Science, EMBO Molecular Medicine, Journal of Neuroinflammation, Molecular Medicine, Frontiers in Immunology, Communications Biology, and others. Research fields span ubiquitin signaling, neuroinflammation, cardiomyopathy, cuproptosis, microRNA regulation, liver disease, pulmonary fibrosis, cervical and head-and-neck cancers, non-small cell lung cancer, dry eye disease, stroke, osteoarthritis, and stem cell biology.

Automatically generated from the 28 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.; Authors - Chunjie Sheng
mouse	WB	Mol Cell; RANK ligand converts the NCoR/HDAC3 co-repressor to a PGC1β- and RNA-dependent co-activator of osteoclast gene expression; Authors - Yohei Abe
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
		Adv Sci (Weinh); Disruption of NF-κB-Mediated Copper Homeostasis Sensitizes Breast Cancer to Cuproptosis; Authors - Xiaomei Zhang
mouse	WB,IF	Adv Sci (Weinh); USP25 Inhibits Neuroinflammatory Responses After Cerebral Ischemic Stroke by Deubiquitinating TAB2; Authors - Zhongding Li
human	WB	J Neuroinflammation; miR-155 and miR-146a collectively regulate meningitic Escherichia coli infection-mediated neuroinflammatory responses.; Authors - Bo Yang