MAP4 Monoclonal antibody, PBS Only

MAP4 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C1A6

Cat no : 68271-1-PBS

Synonyms

DKFZp779A1753, MAP 4, MAP4

---

Validation Data Gallery

• 

  WB analysis using 68271-1-Ig (same clone as 68271-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68271-1-Ig (MAP4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 68271-1-Ig (same clone as 68271-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68271-1-Ig (MAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68271-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68271-1-PBS targets MAP4 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAP4 fusion protein Ag1741
• Full Name: microtubule-associated protein 4
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 210-240 kDa
• GenBank Accession Number: BC012794
• Gene Symbol: MAP4
• Gene ID (NCBI): 4134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)