MAP4K1 Polyclonal antibody

MAP4K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 23950-1-AP

Synonyms

HPK1, MAP4K1, MEK kinase kinase 1, MEKKK 1

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Validation Data Gallery

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  WB analysis of Raji using 23950-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 23950-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 23950-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23950-1-AP (MAP4K1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 23950-1-AP

  IP result of anti-MAP4K1 (IP:23950-1-AP, 4ug; Detection:23950-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 23950-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23950-1-AP (MAP4K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human tonsillitis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

23950-1-AP targets MAP4K1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP4K1 fusion protein Ag20221
• Full Name: mitogen-activated protein kinase kinase kinase kinase 1
• Calculated Molecular Weight: 833 aa, 91 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC152994
• Gene Symbol: MAP4K1
• Gene ID (NCBI): 11184
• RRID: AB_2879372
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K1(Mitogen-activated protein kinase kinase kinase kinase 1) is also named as MEKKK 1, HPK1 and belongs to the protein kinase superfamily. It may function as a novel tumor suppressor and its loss plays a critical role in pancreatic cancer. MAP4K1 regulates stress responses, proliferation, and apoptosis in hematopoietic cells. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Signal; SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.; Authors - Xunping Zhao
human	WB	Clin Exp Med; An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma; Authors - Lin Yang
human	WB	Nat Commun; DNA framework-engineered chimeras platform enables selectively targeted protein degradation; Authors - Li Zhou