MAP4K2 Polyclonal antibody

MAP4K2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 55244-1-AP


BL44, GC kinase, GCK, Germinal center kinase, MAP4K2, MEK kinase kinase 2, MEKKK 2, Rab8 interacting protein, RAB8IP

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, Raji cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55244-1-AP targets MAP4K2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mitogen-activated protein kinase kinase kinase kinase 2
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 85-91 kDa, 58 kDa
GenBank Accession NumberNM_004579
Gene Symbol MAP4K2
Gene ID (NCBI) 5871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.


Product Specific Protocols
WB protocol for MAP4K2 antibody 55244-1-APDownload protocol
IHC protocol for MAP4K2 antibody 55244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols