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MAP4K2 Polyclonal antibody, PBS Only (55244-1-PBS)

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WB analysis of mouse brain using 55244-1-AP (same clone as 55244-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 55244-1-AP (same clone as 55244-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
WB analysis of Raji using 55244-1-AP (same clone as 55244-1-PBS)
Raji cells were subjected to SDS PAGE followed by western blot with 55244-1-AP (MAP4K2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 55244-1-AP (same clone as 55244-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 55244-1-AP (same clone as 55244-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55244-1-AP (MAP4K2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55244-1-PBS in a different storage buffer formulation.

MAP4K2 Polyclonal antibody, PBS Only

Name: MAP4K2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 55244-1-PBS

MAP4K2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55244-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

B lymphocyte serine/threonine-protein kinase, EC:2.7.11.1, GCK, MEKKK 2, Mitogen-activated protein kinase kinase kinase kinase 2

Formulation: PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

55244-1-PBS targets MAP4K2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	mitogen-activated protein kinase kinase kinase kinase 2
Calculated Molecular Weight	92 kDa
Observed Molecular Weight	85-91 kDa, 58 kDa
GenBank Accession Number	NM_004579
Gene Symbol	MAP4K2
Gene ID (NCBI)	5871
RRID	AB_10860908
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12851
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MAP4K2,also named as GCK and RAB8IP, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. It enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. MAP4K2 may play a role in the regulation of vesicle targeting or fusion. This antibody is specific to MAP4K2.