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MAP4K3 Polyclonal antibody

MAP4K3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14702-1-AP

Synonyms

GLK, MAP4K3, MAPKKKK3, MEK kinase kinase 3, MEKKK 3, RAB8IPL1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HeLa cells, human brain tissue
Positive IHC detected inhuman skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14702-1-AP targets MAP4K3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP4K3 fusion protein Ag6372
Full Name mitogen-activated protein kinase kinase kinase kinase 3
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC071579
Gene Symbol MAP4K3
Gene ID (NCBI) 8491
RRIDAB_10858917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4K3(Mitogen-activated protein kinase kinase kinase kinase 3) has kinase activity and that it activates JNK but not MAPK1, MAPK3 or MAPK14 through the activation of MAP3K1 and MAP2K4. Endogenous MAP4K3 kinase activity could be stimulated by exposure to ultraviolet radiation or to tumor necrosis factor.

Protocols

Product Specific Protocols
WB protocol for MAP4K3 antibody 14702-1-APDownload protocol
IHC protocol for MAP4K3 antibody 14702-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB

Bioengineered

Mitogen-activating protein kinase kinase kinase kinase-3, inhibited by Astragaloside IV through H3 lysine 4 monomethylation, promotes the progression of diabetic nephropathy by inducing apoptosis.

Authors - Yuyan Fan
  • KD Validated