MAP7D1 Polyclonal antibody

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  WB analysis using 20788-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20788-1-AP (MAP7D1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 20788-1-AP

  IP result of anti-MAP7D1 (IP:20788-1-AP, 4ug; Detection:20788-1-AP 1:30000) with HEK-293 cells lysate 1470 ug.

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  IHC staining of mouse heart using 20788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 20788-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20788-1-AP (MAP7D1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20788-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U-251 using 20788-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MAP7D1 antibody (20788-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

MAP7D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20788-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Arginine/proline-rich coiled-coil domain-containing protein 1, KIAA1187, MAP7 domain-containing protein 1, PARCC1, PP2464

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Tested Applications

• Positive WB detected in: HEK-293 cells, MDA-MB-231 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse skeletal muscle tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20788-1-AP targets MAP7D1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14385

  Product name: Recombinant human MAP7D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 591-841 aa of BC003083

  Sequence: MAGTTDREEATRLLAEKRRQAREQREREEQERRLQAERDKRMREEQLAREAEARAEREAEARRREEQEAREKAQAEQEEQERLQKQKEEAEARSREEAERQRLEREKHFQQQEQERQERRKRLEEIMKRTRKSEVSETKKQDSKEANANGSSPEPVKAVEARSPGLQKEAVQKEEPIPQEPQWSLPSKELPASLVNGLQPLPAHQENGFSTNGPSGDKSLSRTPETLLPFAEAEAFLKKAVVQSPQVTEVL

• Full Name: MAP7 domain containing 1
• Calculated Molecular Weight: 841 aa, 93 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC003083
• Gene Symbol: MAP7D1
• Gene ID (NCBI): 55700
• RRID: AB_3669347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q3KQU3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP7D1 also known as RPRC1, PARCC1, belongs to the MAP7 family. The MAP7 (Microtubule Associated Protein 7) protein family, consisting of four members, MAP7, MAP7D1, and MAP7D2, MAP7D3, is the microtubule-associated protein involved in various cellular processes regulating microtubule dynamics, organization, and stability(PMID: 28980356). MAP7D1 exhibits the highest conservation with MAP7 and was recently identified as a phosphorylation substrate of DCLK1 in cortical neurons. MAP7D1 is required to maintain MT acetylation, which is enriched in stable MTs(PMID: 35470240). Consistent with the literature, the apparent molecular mass of MAP7D1 detected by Western blot was 120-130 kDa (PMID: 35470240, 37550720).