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JNK Recombinant monoclonal antibody, PBS Only (84875-5-PBS)

JNK Recombinant monoclonal antibody, PBS Only

Name: JNK Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 84875-5-PBS-100UG

JNK Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84875-5-PBS

Clone No. 242364A10

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllIHCIF/ICCFC (Intra)Affinity

IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 84875-5-RR (same clone as 84875-5-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 84875-5-RR (same clone as 84875-5-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 84875-5-RR
Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications
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Product Information

84875-5-PBS targets JNK in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag11785 Product name: Recombinant human MAPK10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-319 aa of BC065516 Sequence: MELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAGPLGCCR Predict reactive species
Full Name	mitogen-activated protein kinase 10
Calculated Molecular Weight	464 aa, 53 kDa
GenBank Accession Number	BC065516
Gene Symbol	JNK3
Gene ID (NCBI)	5602
RRID	AB_3743799
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P53779
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.