JNK Recombinant monoclonal antibody, PBS Only

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  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84875-5-RR (same clone as 84875-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84875-5-RR (MAPK10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84875-5-RR (same clone as 84875-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using JNK antibody (84875-5-RR, Clone: 242364A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84875-5-RR (same clone as 84875-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MAPK10 Recombinant antibody (84875-5-RR, Clone:242364A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84875-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84875-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84875-5-RR against Human MAPK10 were performed. The affinity constant is 2.45 nM.

JNK Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 84875-5-PBS

Clone No.242364A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

MAPK10, 242364A10, c-Jun N-terminal kinase 3, EC:2.7.11.24, JNK1

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Product Information

84875-5-PBS targets JNK in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11785

  Product name: Recombinant human MAPK10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-319 aa of BC065516

  Sequence: MELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGEMVRHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRNYVENRPKYAGLTFPKLFPDSLFPADSEHNKLKASQARDLLSKMLVIDPAKRISVDDALQHPYINVWYDPAEVEAPPPQIYDKQLDEREHTIEEWKELIYKEVMNSEEKTKNGVVKGQPSPSGAAVNSSESLPPSSSVNDISSMSTDQTLASDTDSSLEASAGPLGCCR

• Full Name: mitogen-activated protein kinase 10
• Calculated Molecular Weight: 464 aa, 53 kDa
• GenBank Accession Number: BC065516
• Gene Symbol: JNK3
• Gene ID (NCBI): 5602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P53779
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.