|Positive WB detected in||Jurkat cells, human heart tissue, mouse skeletal muscle tissue|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17376-1-AP targets MAPK11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAPK11 fusion protein Ag11184|
|Full Name||mitogen-activated protein kinase 11|
|Calculated molecular weight||364 aa, 41 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||BC027933|
|Gene ID (NCBI)||5600|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MAPK11(Mitogen-activated protein kinase 11) is also named as PRKM11, SAPK2, SAPK2B,p38b and belongs to the MAP kinase subfamily.It is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors.In mouse brain, both p38a and p38b are present in neurons, while p38b is also expressed in glial cells(PMID:15748168).
Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice.
p38β MAPK signaling mediates exenatide-stimulated microglial β-endorphin expression.
Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.
BMC Endocr Disord
The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.
J Hazard Mater
Metastatic effects of environmental carcinogens mediated by MAPK and UPR pathways with an in vivo Drosophila Model