SAPK4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SAPK4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC

Conjugate

Unconjugated

Publications

3

Cat no : 10217-1-AP

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

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Validation Data Gallery

• 

  WB analysis using 10217-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10217-1-AP (SAPK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10217-1-AP (SAPK4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U-937 cells, HEK-293 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10217-1-AP targets SAPK4 in WB, IHC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SAPK4 fusion protein Ag0255
• Full Name: mitogen-activated protein kinase 13
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC000433
• Gene Symbol: MAPK13
• Gene ID (NCBI): 5603
• RRID: AB_2877727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
hamster	WB	Sci Rep; Cellular response to persistent foot-and-mouth disease virus infection is linked to specific types of alterations in the host cell transcriptome.; Authors - Lingling Han
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang