MAPKAPK3 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 15424-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15424-1-AP (MAPKAPK3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15424-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15424-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15424-1-AP (MAPKAPK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAPKAPK3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15424-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MAPKAP kinase 3, MAPK-activated protein kinase 3, MAP kinase-activated protein kinase 3, EC:2.7.11.1, Chromosome 3p kinase

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

15424-1-AP targets MAPKAPK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7875

  Product name: Recombinant human MAPKAPK3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 179-382 aa of BC001662

  Sequence: KDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFPPFYSNTGQAISPGMKRRIRLGQYGFPNPEWSEVSEDAKQLIRLLLKTDPTERLTITQFMNHPWINQSMVVPQTPLHTARVLQEDKDHWDEVKEEMTSALATMRVDYDQVKIKDLKTSNNRLLNKRRKKQAGSSSASQGCNNQ

• Full Name: mitogen-activated protein kinase-activated protein kinase 3
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001662
• Gene Symbol: MAPKAPK3
• Gene ID (NCBI): 7867
• RRID: AB_2878136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16644
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Bioengineered; Inhibition of translocator protein 18 kDa suppressed the progression of glioma via the ELAV-like RNA-binding protein 1/MAPK-activated protein kinase 3 axis.; Authors - Jingya Wang
rat	WB,IF	Front Neurosci; Identification of key genes and pathways revealing the central regulatory mechanism of brain-derived glucagon-like peptide-1 on obesity using bioinformatics analysis; Authors - Yuwei Shao
human	WB	Nat Commun; Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator; Authors - Na Jiang