MARK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MARK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15492-1-AP

Synonyms

ELKL motif kinase 1, EMK 1, EMK1, MARK2, PAR 1, PAR1 homolog, Par1b

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15492-1-AP (MARK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 15492-1-AP

  IP result of anti-MARK2 (IP:15492-1-AP, 4ug; Detection:15492-1-AP 1:1000) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15492-1-AP (MARK2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15492-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15492-1-AP (MARK2 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human prostate cancer tissue, human brain tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

15492-1-AP targets MARK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MARK2 fusion protein Ag7884
• Full Name: MAP/microtubule affinity-regulating kinase 2
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 77-90 kDa
• GenBank Accession Number: BC008771
• Gene Symbol: MARK2
• Gene ID (NCBI): 2011
• RRID: AB_2140752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARK2 is also named as EMK1(ELKL motif kinase 1), Par1b and belongs to the CAMK Ser/Thr protein kinase family. Par1b/MARK2 plays a critical role in neuronal polarity in the process of axon specification from multiple candidate neurites using primary cultures of mammalian hippocampal neurons(PMID:20194617). It has 16 isoforms produced by alternative promoter usage and alternative splicing with the molecular mass of 77-90kDa and can exsit as a dimer(PMID:16803889).

Publications

Species	Application	Title
human	WB	Mol Cell; An AMPK-Independent Signaling Pathway Downstream of the LKB1 Tumor Suppressor Controls Snail1 and Metastatic Potential.; Authors - Jonathan M Goodwin
human	IP	Nat Commun; TNK1 is a ubiquitin-binding and 14-3-3-regulated kinase that can be targeted to block tumor growth.; Authors - Tsz-Yin Chan ; KD Validated
rat	WB	FASEB J; Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane.; Authors - Ying Gao
human	WB	J Cell Sci; Molecular determinants of αVβ5 localization in flat clathrin lattices - role of αVβ5 in cell adhesion and proliferation.; Authors - Alba Zuidema ; KD Validated
rat	WB	Endocrinology; Basement Membrane Laminin α2 Regulation of BTB Dynamics via Its Effects on F-Actin and Microtubule Cytoskeletons Is Mediated Through mTORC1 Signaling.; Authors - Ying Gao
rat	WB	Endocrinology; CAMSAP2 Is a Microtubule Minus-End Targeting Protein That Regulates BTB Dynamics Through Cytoskeletal Organization.; Authors - Bai-Ping Mao