- Featured Product
- KD/KO Validated
MARK4 Polyclonal antibody
MARK4 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 20174-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human kidney tissue, human colon tissue, human gliomas tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 20174-1-AP is MARK4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MARK4 fusion protein Ag14013 |
| Full Name | MAP/microtubule affinity-regulating kinase 4 |
| Calculated Molecular Weight | 752 aa, 83 kDa |
| GenBank Accession Number | BC009049 |
| Gene Symbol | MARK4 |
| Gene ID (NCBI) | 57787 |
| RRID | AB_2636847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARK4(MAP/microtubule affinity-regulating kinase 4) is also named as KIAA1860, MARKL1 and belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. It is directly involved in microtubule organization in neuronal cells and may contribute to the pathological phosphorylation of tau in Alzheimer's disease and it also plays a role in hepatocellular carcinogenesis. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 83 kDa and 75 kDa. MARK4 can exsit as 40-65 kDa forms in hippocampal homogenates from Alzheimer`s disease and non-demented elderly cases besides the full-length isoform 84 kDa. (PMID:24533944).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MARK4 antibody 20174-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Cdc42 is involved in NC1 peptide-regulated BTB dynamics through actin and microtubule cytoskeletal reorganization. | ||
FASEB J NC1-peptide regulates spermatogenesis through changes in cytoskeletal organization mediated by EB1. | ||
FASEB J Regulation of the blood-testis barrier by a local axis in the testis: role of laminin α2 in the basement membrane. | ||
Endocrinology Sperm Release at Spermiation Is Regulated by Changes in the Organization of Actin- and Microtubule-Based Cytoskeletons at the Apical Ectoplasmic Specialization-A Study Using the Adjudin Model. | ||
Toxicol Sci Regulation of BTB dynamics in spermatogenesis - insights from the adjudin toxicant model. | ||
J Oral Microbiol Microtubule affinity regulating kinase 4 promoted activation of the NLRP3 inflammasome-mediated pyroptosis in periodontitis. |
